Original scientific paper
Clariid Fish Genetic Differentiation and Molecular Phylogenetics Based on the Mitochondrial Cytochrome B Gene from Three Phylo-Geographically Distinct Waterbodies
2022, 80 (3) p. 0-0
Abstract
The population architecture and genetic variability of Clarias gariepinus (Burchell, 18) were investigated using partial mitochondrial DNA cytochrome b region sequences. Fifty-four (54) samples were investigated from three geographically isolated rivers in Nigeria. The analysis of 53 haplotypes revealed greater haplotype diversity (0.99930) and nucleotide diversity (p) (0.07270). According to an analysis of molecular variance (AMOVA), the genetic diversity of Clarias gariepinus within populations is significantly higher than the genetic diversity across populations. The FST scores (0.95699 to 0.7500) indicated that Clarias gariepinus populations in three Nigerian freshwater bodies had a strong genetic structure. The phylogenetic reconstruction of unique haplotypes revealed the placement of a haplotype (Ogbese) linked by others from all three groups with a point mutation ranging from 1–24 nucleotides. C. gariepinus populations in Asejire and Ureje are genetically diverse, as evidenced by a high level of haplotype diversity of 1.0000, low nucleotide diversity spanning from 0.05101 to 0.07889, and high FST values (within population genetic variation). The common haplotypes between some populations and mixes of haplotypes from different populations within the same genetic cluster demonstrate that population genetic structure is not distinct.
Keywords
Clarias gariepinus, Cytochrome b, median-joining network, Nucleotide Substitution, genetic distances