Original scientific paper
DNA Barcoding Approach: Assessing Diversity of Economically Important Anchovy in the North Coast of Java, Indonesia
2023, 81 (2) p. 0-0
Wiwiet Taufani, Anhar Solichin, Suradi Saputra, Diah Ayuningrum
Abstract
Anchovy is among the economically important fish in the western part of Central Java North Coastal. The production of anchovies expands along the coastal of Brebes Regency to Batang Regency. Therefore, this study aims to determine the diversity of anchovies in the northern part of the Central Java North Coastal (Brebes, Tegal, Pemalang, Batang, and Kendal) using a molecular approach. This study was conducted from April to September 2019 in the Laboratory of Tropical Marine Biotechnology, Faculty of Fisheries and Marine Science, Universitas Diponegoro. The descriptive explorative method was applied in this study with a random sampling technique, while the Cytochrome Oxidase I (COI) gene was used to identify the molecular. An anchovy sample identified molecularly from each region was found from the sampling, including BB 3, PML 2, BTG 1, BTG 2, BTG 4, TGL 2, TGL 4, KDL 1, and KDL 4. The base-pair length of 10 anchovy samples from the COI gene amplification result was at 600-700bp. The sequencing and alignment results in the BLAST feature (https://www.ncbi.nlm.nih.gov/) showed that the ten Anchovy samples were included in the type of Encrasicholina heteroloba, Stolephorus commersoni, Stolephorus waitei and Atherinomorus sp., which similarity level ranged from 98-99% in each location. Therefore, it is concluded that at least four different species of anchovy existed on the North Coast of Java. However, further research is suggested to determine the genetic variation on each species for better fisheries management.
Keywords
Anchovy, molecular, identification, COI, North Coast of Java