Original scientific paper
Genetic Analysis of Three Geographically Secluded Populations of Oreochromis niloticus (Cichlidae) Based on Mitochondrial DNA Cytochrome b
2024, 82 (2) p. 0-0
Abstract
A significant fisheries resource and a valuable species in aquaculture programmes is the fish species Oreochromis niloticus, which is found almost everywhere and is widely dispersed throughout all River bodies. Several natural populations of this species have been impacted by genetic pollution despite their significant economic relevance. Understanding its population structure is a crucial first step in protecting this species in their native habitats and in choosing which wild stocks to use in hatchery initiatives. To understand the genetic-population structure of this species, The genetic differences among three geographically secluded populations of O. niloticus were investigated utilizing mitochondrial DNA Cyt b (Cytochrome b) gene sequences. The results were used to estimate the levels of within and among population genetic variability. The 56 Cyt b (821 bp) sequences analysis revealed 21 haplotypes, with 0.0510 nucleotide diversity and 0.881 haplotypes diversity, with 7 sites with Singleton variables and informative parsimony 19 in each of the populations, genetic diversity could be identified, and only a few population haplotypes were found, which points to a minor genetic distinction between them. For purposes of conservation and/or aquaculture programmes, the gathered information can help choose fish populations that maintain a greater genetic variation in O. niloticus.
Keywords
Genetic characterization, mitochondria DNA, Cytochrome b, Nile tilapia, Nucleotide frequencies